张元兴
生物工程专业：博士生导师/硕士生导师
生物化工专业：博士生导师/硕士生导师
生物工程领域：工程硕士生导师
1982年1月毕业于华东化工学院化学工程系，获学士学位。1984年7月毕业于华东化工学院生物化工系，获硕士学位，1995年9月评为4688美高梅集团唯一教授。1997年担任博士生导师。1998年享受国务院政府特殊津贴。
曾任国家863高技术计划海洋技术领域专家组副组长，国务院学位委员会生物工程临时学科评议组专家，4688美高梅集团唯一院长。受聘为国家海洋食品工程技术研究中心专家指导委员会副主任，福建省海洋功能食品工程技术研究中心、福建省海产品废弃物综合利用工程技术研究中心技术委员会主任，山东省水产动物免疫制剂工程研究中心专家委员会主任，福建省海洋生物资源开发利用协同创新中心学术委员会副主任，农业部水产养殖病害防治专家委员会顾问委员，海洋生物制备技术国家地方联合工程实验室、教育部海水养殖重点实验室、教育部水产种质资源发掘与利用重点实验室、教育部海洋生物遗传学与育种重点实验室、农业部海洋渔业资源可持续发展重点实验室、农业部海洋与河口渔业资源及生态重点开放实验室、农业部东海海水健康养殖重点实验室、农业部东海与远洋渔业资源开发利用重点实验室、农业部渔业装备与工程技术重点实验室、农业部海水养殖病害防治重点实验室、广东省水产经济动物病原生物学及流行病学重点实验室学术委员会委员，上海海洋药物工程技术研究中心技术委员会委员。担任Bioresouces and Bioprocessing、中国抗生素杂志、中国海洋药物编委。目前承担国家自然科学基金重点项目。为本科生讲授上海市精品课程生物化学，为博士研究生开设生物反应器设计与放大课程，已指导69名硕士研究生和52名博士研究生毕业，8名博士后出站，正在指导硕士研究生6名，博士研究生8名。已在国内外发表论文460余篇，其中SCI收录247篇，出版著作2部，参编著作6部，获省部级科技进步奖6项，教学成果奖1项，中国石油和化学工业优秀科技图书一等奖1项，申请国家发明专利70项，其中获得授权54项；申请国际专利3项，其中获得授权美国专利2项。
学术梯队：
1.周祥山，博士，教授，博士生导师，上海市科技启明星，山东省泰山学者。参加国家自然科学基金项目，主要从事微生物发酵和生物制药的研究。
2.刘琴，博士，教授，博士生导师，国家优秀青年基金获得者，上海市科技启明星，教育部新世纪人才，任生物反应器工程国家重点实验室副主任，阿华生物工程研究所所长，上海海洋动物疫苗工程技术研究中心主任。承担国家重点研发计划项目、国家自然科学基金项目，主要从事海洋生物技术研究。
3.王启要，博士，教授，博士生导师，上海市科技启明星和浦江学者，任4688美高梅集团唯一副院长。国家鲆鲽类农业技术体系疾病防控岗位科学家。承担国家自然科学基金项目，参加国家重点研发计划课题，主要从事海洋生物技术研究。
4.蔡孟浩，博士，副教授，硕士生导师，上海市科技启明星。承担国家重点研发计划课题、国家自然科学基金和上海市自然科学基金项目，主要从事海洋微生物和合成生物学的研究。
5.阳大海，博士，副教授，硕士生导师，上海市浦江学者和晨光学者，入选中国科协青年人才托举计划。承担国家自然科学基金项目，参加国家重点研发计划课题，主要从事海洋生物技术研究。
6.马悦，博士。承担国家自然科学基金项目，参加国家重点研发计划课题和国家鲆鲽类农业技术体系项目，主要从事海洋生物技术研究。
7.刘晓红，博士。承担国家自然科学基金项目，参加国家重点研发计划课题，主要从事海洋动物疫苗的研究。
8.殷开宇，博士后。参加国家自然科学基金项目，主要从事海洋生物技术的研究。
9.陆健，学士，高级工程师。主要从事生物反应器的研究和设计。
10.林雅惠，学士。实验研究和实验室管理工作。
主要的研究方面：
1.海洋生物技术。研究海洋病原微生物的特性，掌握其致病规律，在分子水平上阐明细胞通讯相关的条件致病现象，研究病原细菌和宿主之间的互作机制，从基因水平上进行改造，开发减毒活疫苗和多价疫苗。目前承担着国家自然科学基金重点和面上项目、现代农业产业技术体系岗位科学家项目。
2.微生物工程。研究甲醇酵母基因诱导水平的精密控制网络，构建新的蛋白表达体系。解析生物合成代谢途径和相关基因（簇），在毕赤酵母中重构代谢网络，高效异源生物合成小分子活性化合物。在海洋微生物培养中，阐明丝状菌次级代谢调控的规律，调控菌丝形态，高效合成活性化合物。目前承担着国家自然科学基金项目和企业委托项目。
3.动物细胞培养。研究动物细胞的生理和代谢规律，开发无血清培养基，建立动物细胞培养生产蛋白药物的优化工艺。研究动物细胞表达外源基因的高效体系，构建适合于大规模无血清培养的重组细胞株。目前承担着企业委托项目。
著作：
1、王启要，刘琴，张元兴 (2008)群体感应：细菌之间的语言.叶勤主编：生物科学与工程.北京：科学出版社. p43-67.
2、张元兴 (2008)动物细胞培养工程.叶勤主编：生物科学与工程.北京：科学出版社. p160-171.
3、张元兴，易小萍，张立，孙祥明 (2007)动物细胞培养工程.北京：化工出版社.
4、张元兴，许学书 (2001)生物反应器工程.上海：4688美高梅集团唯一出版社.
2018年发表和出版中论文:
1.Pengcheng Bao, Xiang Sun, Qin Liu,Yuanxing Zhang, Xiaohong Liu*. Synergistic effect of a combined liveVibrio anguillarum andEdwardsiella piscicida vaccine in turbot. Fish & Shellfish Immunology, accepted.
2.Ying Wen, Shouwen Chen, Zhiwei Jiang, Zhuang Wang, Jinchao Tan, Tianjian Hu, Qiyao Wang, Xiangshan Zhou,Yuanxing Zhang, Qin Liu, Dahai Yang*. Dysregulated hemolysin promotes bacterial outer membrane vesicles-induced pyroptotic-like cell death in zebrafish. Cellular Microbiology, accepted.
3.Zhuang Wang, Lingyun Lin, Weijie Chen, Xin Zheng,Yuanxing Zhang, Qin Liu, Dahai Yang*. Neutrophil plays critical role duringEdwardsiella piscicida immersion infection in zebrafish larvae. Fish & Shellfish Immunology 2019,87: 565-572.
4.Tianjian Hu, Lingzhi Zhang, Wei Wang, Dahai Yang, Jingfan Xiao,Yuanxing Zhang, Xiaohong Liu*, Qin Liu*.Edwardsiella piscicida enters non-phagocytic cells via a macropinocytosis-involved hybrid mechanism. Journal of Bacteriology 2019,201(5): e00548-18.
5.Lifan Wei, Haoxian Qiao, Bing Liu, Kaiyu Yin, Yanyan Wu, Xiaohong Liu, Jingfan Xiao,Yuanxing Zhang, Yue Ma*, Qiyao Wang*. MarTrack: A versatile toolbox ofmariner transposon derivatives used for functional genetic analysis of bacterial genomes. Microbiological Research 2019,219: 84-93.
6.Lei Shi, Jinjia Wang, Xiaolong Wang,Yuanxing Zhang, Zhiwei Song, Menghao Cai, Xiangshan Zhou*. Transcriptome andmetabolomeanalyses reveal global behavior of a genetically engineeredmethanol-independentPichia pastoris strain. Process Biochemistry 2019,76: 46-54.
7.Mingyu Hou, Wenhui Wang, Feizi Hu,Yuanxing Zhang, Dahai Yang*, Qin Liu*. Phosphothreonine lyase promotes p65 degradation in a mitogen-activated protein kinase/mitogen- and stress-activated protein kinase 1-dependent manner. Infection and Immunity 2019,87(1): e00508-18.
8.Wenting Xu, Chenglong Jiao, Pengcheng Bao, Qin Liu, Pengbo Wang, Ruilin Zhang,Yuanxing Zhang, Xiaohong Liu*. Efficacy of MontanideTM ISA 763 A VG as aquatic adjuvant administrated with an inactivatedVibrio harveyi vaccine in turbot (Scophthalmus maximus L.). Fish and Shellfish Immunology 2019,84: 56-61.
9.Xiaoyue Sun, Wei Shen, Yanyun Gao, Menghao Cai, Mian Zhou*,Yuanxing Zhang. Heterologous expression and purification of a marine alginate lyase inEscherichia coli. Protein Expression and Purification 2019,153: 97-104.
10.Jinxiang Zhu, Qiaoyun Zhu, Ruiqing Gong, Qin Xu, Menghao Cai, Tianyi Jiang, Xiangshan Zhou, Mian Zhou*,Yuanxing Zhang.PiggyBac transposon-mediated mutagenesis and application in yeastKomagataella phaffii. Biotechnology Letters 2018,40(9-10): 1365-1376.
11.Guanhua Yang, Cheng Wang, Xuetong Wang, Ruiqing Ma, Huajun Zheng, Qin Liu,Yuanxing Zhang, Yue Ma*, Qiyao Wang*. Complete genome sequence of the marine fish pathogenVibrio anguillarum and genome-wide transposon mutagenesis analysis of genes essential forin vivo infection. Microbiological Research 2018,216: 97-107.
12.Wenting Xu, Zhaoyan Gu, Lingzhi Zhang,Yuanxing Zhang, Qin Liu, Dahai Yang*.Edwardsiella piscicida virulence effector Trxlp promotes the NLRC4 inflammasome activation during infection. Microbiol Pathogenesis 2018,123: 496-504.
13.Huifang Cao, Fajun Han, Jinchao Tan, Mingyu Hou,Yuanxing Zhang, Dahai Yang*, Qin Liu*.Edwardsiella piscicida type III secretion system effector EseK inhibits mitogen-activated protein kinase phosphorylation and promotes bacterial colonization in zebrafish larvae. Infection and Immunity 2018,86(9): e00233-18.
14.Kaiyu Yin, Yunpeng Guan, Ruiqing Ma, Lifan Wei, Bing Liu, Xiaohong Liu, Xiangshan Zhou, Yue Ma,Yuanxing Zhang, Matthew K. Waldor, Qiyao Wang*. Critical role for a promoter discriminator in RpoS control of virulence inEdwardsiella piscicida. PLoS Pathogens 2018,14(8): e1007272.
15.Shouwen Chen, Dahai Yang, Ying Wen, Zhiwei Jiang, Lingzhi Zhang, Jiatiao Jiang, Yaozhen Chen, Tianjian Hu, Qiyao Wang,Yuanxing Zhang, Qin Liu*. Dysregulated hemolysin liberates bacterial outer membrane vesicles for cytosolic lipopolysaccharide sensing. PLoS Pathogens 2018,14(8): e1007240.
16.Dahai Yang, Xin Zheng, Shouwen Chen, Zhuang Wang, Wenting Xu, Jinchao Tan, Tianjian Hu, Mingyu Hou, Wenhui Wang, Zhaoyan Gu, Qiyao Wang, Ruilin Zhang,Yuanxing Zhang, Qin Liu*. Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish. Nature Communications 2018,9: 3052.
17.Ying Lou, Guorong Ji, Qin Liu, Pengbo Wang, Ruilin Zhang,Yuanxing Zhang, Xiaohong Liu*. Secretary expression and scale-up production of recombinant human thyroid peroxidase via baculovirus/insect cell system in a wave-like bioreactor. Protein Expression and Purification 2018,149: 7-12.
18.李佳欣,刘绍梅,任燕娜,周祥山,蔡孟浩*,张元兴.海洋灰绿曲霉AgveA基因响应盐度胁迫的功能分析.4688美高梅集团唯一学报2018,44(5): 28-38.
19.陈军军,娄颖,张元兴,刘琴,刘晓红*.增殖细胞核抗原蛋白在Spodoptera frugiperda昆虫细胞中的表达及纯化.中国生物工程杂志2018,38(7): 14-20.
20.inxiang Zhu, Ruiqing Gong, Qiaoyun Zhu, Qiulin He, Ning Xu, Yichun Xu, Menghao Cai, Xiangshan Zhou,Yuanxing Zhang, Mian Zhou*. Genome-wide determination of gene essentiality by transposon insertion sequencing in yeastPichia pastoris. Scientific Reports 2018,8: 10223.
21.Lifan Wei, Yanyan Wu, Haoxian Qiao, Wensheng Xu,Yuanxing Zhang, Xiaohong Liu*, Qiyao Wang*. YebC controls virulence by activating T3SS gene expression in the pathogenEdwardsiella piscicida. FEMS Microbiology Letters 2018,365(14): fny137.
22.Yiqi Liu, Chenxiao Bai, Qin Xu, Jiahui Yu, Xiangshan Zhou,Yuanxing Zhang, Menghao Cai*. Improved methanol-derived lovastatin production through enhancement of the biosynthetic pathway and intracellular lovastatin efflux inmethylotrophic yeast. Bioresouces and Bioprocessing 2018,5: 22.
23.Xiating Gao, Xuetong Wang, Qiaoqiao Mao, Rongjing Xu, Xiaohui Zhou, Yue Ma, Qin Liu,Yuanxing Zhang, Qiyao Wang*. VqsA, a novel LysR-type transcriptional regulator coordinates quorum sensing (QS) and is controlled by QS to regulate virulence in the pathogenVibrio alginolyticus. Applied and Environmental Microbiology 2018,84(12): e00444-18.
24.Ya Zhou, Jingfan Xiao*, Xin Ma, Qiyao Wang,Yuanxing Zhang. An effective established biosensor of bifunctional probes-labeled AuNPs combined with LAMP for detection of fish pathogenStreptococcus iniae. Applied Microbiology and Biotechnology 2018,102(12): 5299-5308.
25.Ning Xu, Jinxiang Zhu, Qiaoyun Zhu, Yanzi Xing, Menghao Cai, Tianyi Jiang, Mian Zhou*,Yuanxing Zhang. Identification and characterization of novel promoters for recombinant protein production in yeastPichia pastoris. Yeast 2018,35(5): 379-385.
26.Yunpeng Guan, Kaiyu Yin, Mian Zhou, Minjun Yang,Yuanxing Zhang, Xiaohong Liu*, Qiyao Wang*. EsrB negatively regulates expression of the glutamine sythetase GlnA in the fish pathogenEdwardsiellapiscicida. FEMS Microbiology Letters 2018,365(4): fny007.
27.Lei Shi, Xiaolong Wang, Jinjia Wang, Ping Zhang, Fei Qi, Menghao Cai*,Yuanxing Zhang, Xiangshan Zhou*. Transcriptome analysis ofDmig1Dmig2 mutant reveals their roles in methanol catabolism, peroxisome biogenesis and autophagy in methylotrophic yeastPichia pastoris. Genes & Genomics 2018,40(4): 399-412.
28.Zhen Yang, Xuetong Wang, Wensheng Xu, Mian Zhou,Yuanxing Zhang, Yue Ma*, Qiyao Wang*. Phosphorylation of PppA at threonine 253 controls T6SS2 expression and bacterial killing capacity in the marine pathogenVibrio alginolyticus. Microbiological Research 2018,209: 70-78.
29.Xiaohong Liu, Chenglong Jiao, Yue Ma*, Qiyao Wang,Yuanxing Zhang. A live attenuatedVibrio anguillarum vaccine induces efficient immunoprotection in Tiger puffer (Takifugu rubripes). Vaccine 2018,36(11): 1460-1466.
30.Zhen Yang, Xiaohui Zhou, Yue Ma, Mian Zhou, Matthew K. Waldor,Yuanxing Zhang, Qiyao Wang*. Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogenVibrio alginolyticus. Environmental Microbiology 2018,20(2): 903-909.
31.Lingzhi Zhang, Zhiwei Jiang, Shan Fang, Yajun Huang, Dahai Yang, Qiyao Wang,Yuanxing Zhang, Qin Liu*. Systematic identification of intracellular-translocated candidate effectors inEdwardsiella piscicida. Frontiers in Cellular and Infection Microbiology 2018,8: 37.
32.Chuixing Kong, Hezhou Huang, Ying Xue, Yiqi Liu, Qiangqiang Peng, Qi Liu, Qin Xu, Qiaoyun Zhu, Ying Yin, Xiangshan Zhou,Yuanxing Zhang, Menghao Cai*. Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis. Scientific Reports 2018,8: 2116.
33.Jinjia Wang, Xiaolong Wang, Lei Shi,Yuanxing Zhang, Xiangshan Zhou*,Menghao Cai*. Reduced methanol input induces increased protein output byAOX1 promoter in atrans-acting elements engineeredPichia pastoris. Journal of Industrial Microbiology and Biotechnology 2018,45(1): 25-30.
34.Yiqi Liu, Xiaohu Tu, Qin Xu, Chenxiao Bai, Chuixing Kong, Qi Liu, Jiahui Yu, Qiangqiang Peng, Xiangshan Zhou,Yuanxing Zhang, Menghao Cai*. Engineering monoculture and co-culture of methylotrophic yeast forde novo production of monacolin J and lovastatin from methanol. Metabolic Engineering 2018,45: 189-199.
35.Huifang Cao, Cuiting Yang, Shu Quan, Tianjian Hu, Lingzhi Zhang,Yuanxing Zhang, Dahai Yang*, Qin Liu*. Novel T3SS effector EseK inEdwardsiella piscicida is chaperoned by EscH and EscS to express virulence. Cellular Microbiology 2018,20(1): e12790.
2017年以前发表的主要文章:
1.Yuan Wu, Yanna Ren, Xiangshan Zhou, Menghao Cai*,Yuanxing Zhang*. Transcription factorAgseb1 affects development, osmotic stress response, and secondary metabolism in marine-derived Aspergillus glaucus. Journal of Basic Microbiology 2017,57(10): 873-882.
2.Guanhua Yang, Gabriel Billings, Troy Hubbard, Joseph Park, Ka Yin Leung, Qin Liu, Brigid M. Davis,Yuanxing Zhang, Qiyao Wang*, Matthew K. Waldor. Time resolved transposon insertion sequencing reveals genome-wide fitness dynamics during infection. mBio 2017,8(5): e01581-17.
3.Yue Ma, Qiyao Wang*, Wensheng Xu, Xiaohong Liu, Xiating Gao,Yuanxing Zhang*. Stationary phase-dependent accumulation of ectoine is an efficient adaptation strategy inVibrio anguillarum against cold stress. Microbiological Research 2017,205: 8-18.
4.Ying Yin, Qiang Fu, Wenhui Wu, Menghao Cai, Xiangshan Zhou,Yuanxing Zhang*. Producing novel fibrinolytic isoindolinone derivatives in marine fungusStachybotrys longisporaFG216 by rational amino precursors supply according to its biosynthesis pathway. Marine Drugs 2017,15(7): 214.
5.Hao Chen, Dahai Yang, Fajun Han, Jinchao Tan, Lingzhi Zhang, Jingfan Xiao,Yuanxing Zhang, Qin Liu*. The bacterial T6SS effector EvpP prevents NLRP3 inflammasome activation by inhibiting Ca2+-dependent MAPK-Jnk pathway. Cell Host & Microbe 2017,21(1): 47-58.
6.Yue Ma, Qiyao Wang, Xiating Gao,Yuanxing Zhang*. Biosynthesis and uptake of glycine betaine as cold-stress response to low temperature in fish pathogenVibrio anguillarum. Journal of Microbiology 2017,55(1): 44-55.
7.Ying Yin*, Menghao Cai, Xiangshan Zhou, Zhiyong Li,Yuanxing Zhang*. Polyketides inAspergillus terreus: biosynthesis pathway discovery and application. Applied Microbiology and Biotechnology 2016,100(18): 7787-7798.
8.Dan Gu, Min Guo, Minjun Yang,Yuanxing Zhang, Xiaohui Zhou*, Qiyao Wang*. AsE-mediated temperature gauge controls a switch from LuxR-mediated virulence gene expression to thermal stress adaptation inVibrio alginolyticus. PLoS Pathogens 2016,12(6): e1005645.
9.Xiaolong Wang, Qi Wang, Jinjia Wang, Peng Bai, Lei Shi, Wei Shen, Mian Zhou, Xiangshan Zhou,Yuanxing Zhang, Menghao Cai*. Mit1 transcription factor mediates methanol signaling and regulatesalcohol oxidase 1 promoter inPichia pastoris. Journal of Biological Chemistry 2016,291(12): 6245-6261.
10.Tongcheng Dai, Na Li, Fajun Han, Hua Zhang,Yuanxing Zhang, Qin Liu*. AMP-guided tumour-specific nanoparticle delivery via adenosine A1 receptor. Biomaterials 2016,83: 37-50.
11.John S. Y. Goh, Yingwei Liu, Haifeng Liu, Kah Fai Chan, Corrine Wan, Gavin Teo, Xiangshan Zhou, Fusheng Xie, Peiqing Zhang,Yuanxing Zhang*, Zhiwei Song* (2014) Highly sialylated recombinant human erythropoietin production in large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with restored GnT I function. Biotechnology Journal9(1): 100-109.
12.Pham Ngoc Tu, Yamin Wang, Menghao Cai, Xiangshan Zhou*,Yuanxing Zhang* (2014) Co-expressions of protein tyrosine kinases EGFR-2 and PDGFRβ with protein tyrosine phosphatase 1B inPichia pastoris. Journal of Microbiology and Biotechnology24(2): 152-159.
13.Yingwei Liu, Xiangshan Zhou, Zhiwei Song*,Yuanxing Zhang* (2014) Sodium butyrate enhances the acidic isoform content of recombinant human erythropoietin produced by Chinese hamster ovary cells. Biotechnology Letters36(5): 907-911.
14.Haifeng Liu, Xiangshan Zhou*, Shousheng Tian, Xianghui Hao, Jinhua You,Yuanxing Zhang* (2014) Two-step transpeptidation of the insulin precursor expressed inPichia pastoris to insulin ester via trypsin-catalyzed cleavage and coupling. Biotechnology and Applied Biochemistry61(4): 408-417.
15.Lili Sheng, Yuanzhi Lv, Qin Liu,Qiyao Wang*,Yuanxing Zhang* (2013) Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogenVibrio alginolyticus through phosphatase PppA. Veterinary Microbiology162(2-4): 652-662.
16.Haifeng Liu, Xiangshan Zhou*, Fusheng Xie, Jinhua You,Yuanxing Zhang* (2013)An efficient trypsin digestion strategy for improving desB30 productivity from recombinant human insulin precursor fusion protein. Process Biochemistry48(5-6): 965-971.
17.Huan Liu, Dan Gu, Xiaodan Cao, Qin Liu,Qiyao Wang*,Yuanxing Zhang* (2012) Characterization of a new quorum sensing regulatorluxT and its roles in the extracellular protease production, motility, and virulence in fish pathogenVibrio alginolyticus. Archives of Microbiology194(6): 439-452.
18.Ming Liu, Shuang Wang, Tingguang Sun, Jiguo Su,Yuanxing Zhang*, Junjie Yue*,Zhiwei Sun* (2012) Insight into the structure, dynamics and the unfolding property of amylosucrases: Implications of rational engineering on thermostability. PLoS One7(7): e40441.
19.Dahai Yang, Qin Liu*, Mingjun Yang, Haizhen Wu,Qiyao Wang, Jingfan Xiao,Yuanxing Zhang* (2012) RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish. BMC Genomics13: 319.
20.Menghao Cai, Xiangshan Zhou*, Jian Lu, Weimin Fan, Jiushun Zhou, Chuanpeng Niu, Li Kang, Xunqian Sun,Yuanxing Zhang* (2012) An integrated control strategy for the fermentation of the marine-derived fungus Aspergillus glaucus for the production of anti-cancer polyketide. Marine Biotechnology14(6): 665-671.
21.Menghao Cai, Xueqian Sun, Xiangshan Zhou*,Yuanxing Zhang* (2012) Roles of cobalt in biosynthesis stimulation of a cytotoxic compound from marine-derivedAspergillus glaucus. Process Biochemistry47(12): 2267-2274.
22.Menghao Cai, Xiangshan Zhou*, Jian Lu, Weimin Fan, Chuanpeng Niu, Jiushun Zhou, Xueqian Sun, Li Kang,Yuanxing Zhang* (2011) Enhancing aspergiolide A production from a shear-sensitive and easy-foaming marine-derived filamentous fungusAspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor. Bioresource Technology102(3): 3584-3586.
23.Xiaodan Cao,Qiyao Wang, Qin Liu*, Haopeng Rui, Huan Liu,Yuanxing Zhang* (2011) Identification of aluxO-regulated extracellular protein Pep and its roles in motility inVibrio alginolyticus. Microbial Pathogenesis50(2):123-131.
24.Lu Cheng, Xiangming Sun, Xiaoping Yi,Yuanxing Zhang* (2011) Transient gene expression of a mouse homolog of Fce-Fcg fusion protein for anti-allergic function assay. Process Biochemistry46(4): 858-863.
25.XinWang, Qiyao Wang*, Minjun Yang, Jingfan Xiao, Qin Liu, Haizhen Wu,Yuanxing Zhang* (2011) QseBC controls flagellar motility, fimbrial hemagglutination and intracellular virulence in fish pathogenEdwardsiella tarda. Fish & Shellfish Immunology30(3): 944-953.
26.Honghong He,Qiyao Wang, Lili Sheng, Qin Liu*,Yuanxing Zhang* (2011) Functional characterization ofVibrio alginolyticus twin-arginine translocation system: its roles in stress adaptation and virulence towards fish. Current Microbiology62(4): 1193-1199.
27.Yanhua Pan, WeiWei, Hongyan Xu,Qiyao Wang, Qin Liu, Haizhen Wu*,Yuanxing Zhang* (2011) Differential gene expression in Japanese flounder (Paralichthys olivaceus) induced by live attenuatedVibrio anguillarum. Aquaculture Research42(7):1042-1049.
28.Huan Liu,Qiyao Wang*, Qin Liu, Xiaodan Cao, Cunbin Shi,Yuanxing Zhang* (2011) Roles of Hfq in the stress adaptation and virulence in fish pathogenVibrio alginolyticus and its potential application as a target for live attenuated vaccine. Applied Microbiology and Biotechnology91(2):353-364.
29.Lu Cheng, Xiangming Sun, Xiaoping Yi,Yuanxing Zhang* (2011) Large-scale plasmid preparation for transient gene expression. Biotechnology Letters33(8): 1559-1564.
30.Yan Zhao, Qin Liu*, Xiuhua Wang, Lingyun Zhou,Qiyao Wang,Yuanxing Zhang* (2011) Surface display ofAeromonas hydrophila GAPDH in attenuatedVibrio anguillarum to develop a novel multivalent vector vaccine. Marine Biotechnology13(5):963-970.
31.Jingfan Xiao, Tao Chen,Qiyao Wang*, Qin Liu, Xin Wang, Yuanzhi Lv, Haizhen Wu,Yuanxing Zhang* (2011) Search for live attenuated vaccine candidate against edwardsiellosis by mutating virulence-related genes of fish pathogenEdwardsiella tarda. Letters in Applied Microbiology53(4): 430-437.
32.Xiaonuo Teng, Xiaoping Yi, Xiangming Sun,Yuanxing Zhang* (2011) Modeling and application of controlled-fed perfusion culture of CHO cells in a bioreactor. Chemical and Biochemical Engineering Quarterly25(3): 385-394.
33.Wei Wei, Haizhen Wu*,Hongyan Xu, Teng Chu, Changwen Wu, Kangmei Chang,Yuanxing Zhang* (2010) Cloning and molecular characterization of lycC1INH genes in large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology28(1): 221-227.
34.Xueqian Sun, Xiangshan Zhou*, Menghao Cai, Jiushun Zhou,Yuanxing Zhang* (2010)Significant stimulation of o-phthalic acid in biosynthesis of aspergiolide A by a marine fungusAspergillus glaucus. Bioresource Technology101(10):3609-3616.
35.Lingyun Zhou, Xiuhua Wang, Qin Liu*,Qiyao Wang, Yan Zhao,Yuanxing Zhang* (2010) A novel multivalent vaccine based on secretary antigen-delivery induces protective immunity againstVibrio anguillarum andAeromonas hydrophila. Journal of Biotechnology146(1-2):25-30.
36.Haopeng Rui, Jennifer M. Ritchie, Roderick T. Bronson, John J. Mekalanos,Yuanxing Zhang, Matthew K. Waldor* (2010) Reactogenicity of live-attenuatedVibrio cholerae vaccines is dependent on flagellins. Proceedings of theNationalAcademy of Sciences of theUnited States of America107(9):4359-4364.
37.Xiaodan Cao,Qiyao Wang, Qin Liu*, Huan Liu, Honghong He,Yuanxing Zhang* (2010) Vibrio alginolyticus MviN is a LuxO-regulated protein and affects cytotoxicity toward EPC cell. Journal of Microbiology and Biotechnology20(2): 271-280.
38.Xiao Zhang, Wei Wei, Haizhen Wu*,Hongyan Xu, Kangmei Chang,Yuanxing Zhang* (2010) Gene cloning and characterization of ferritin H and M subunits from large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology28(5-6):735-742.
39.Menghao Cai, Xiangshan Zhou*, Jiushun Zhou, Chuanpeng Niu, Xueqian Sun,Yuanxing Zhang* (2010) Efficient strategy for enhancing aspergiolide A production by citrate feedings and its effects on sexual development and growth of marine-derived fungusAspergillus glaucus. Bioresource Technology101(15):6059-6068.
40.Cuihong Du, Xiaoping Yi,Yuanxing Zhang* (2010) Expression and purification of soluble recombinant human endostatin inEscherichia coli. Biotechnology and Bioprocess Engineering15(2):229-235.
41.Ping Zhang, Wenwen Zhang, Xiangshan Zhou*, Peng Bai, James M. Cregg,Yuanxing Zhang* (2010) Catabolite repression of Aox inPichia pastoris is dependent on hexose transporter PpHxt1 and pexophagy. Applied and Environmental Microbiology76(18): 6108-6118.
42.XinWang, Qiyao Wang, Jingfan Xiao, Qin Liu*, Haizhen Wu,Yuanxing Zhang* (2010) Hemolysin EthA inEdwardsiella tarda is essential for fish invasionin vivo andin vitro and regulated by two-component system EsrA-EsrB and nucleoid protein HhaEt. Fish and Shellfish Immunology29(6):1082-1091.
43.Suxia Li, Haizhen Wu, Jian Zhao, Ling Ou,Yuanxing Zhang* (2010) A biochemistry and molecular biology experiment and evaluation system for biotechnology specialty students. Biochemistry and Molecular Biology Education38(4): 271-275.
44.Lili Xu,Qiyao Wang, Jingfan Xiao, Qin Liu*, Xin Wang, Tao Chen,Yuanxing Zhang* (2010)Characterization ofEdwardsiella tarda waaL: roles in lipopolysaccharide biosynthesis, stress adaptation, and virulence toward fish. Archives of Microbiology192(12):1039-1047.
45.Qiyao Wang, Minjun Yang, Jingfan Xiao, Haizhen Wu, Xin Wang, Yuanzhi Lv, Lili Xu, Huajun Zheng, Shengyue Wang, Guoping Zhao, Qin Liu*,Yuanxing Zhang* (2009) Genome sequence of the versatile fish pathogenEdwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. PLoS One4(10): e7646.
46.Haopeng Rui, Qin Liu,Qiyao Wang, Yue Ma, Huan Liu,Cunbin Shi,Yuanxing Zhang* (2009) Role of alkaline serine protease Asp inVibrio alginolyticus virulence and regulation of its expression by LuxO-LuxR regulatory system. Journal of Microbiology and Biotechnology19(5): 431-438.
47.Wei Wei, Haizhen Wu,Hongyan Xu*, Tao Xu, Xiao Zhang, Kangmei Chang,Yuanxing Zhang* (2009) Cloning and molecular characterization of two complement Bf/C2 genes in large yellow croaker (Pseudosciaena crocea). Fish & Shellfish Immunology27(2):285-295.
48.XinWang, Qiyao Wang*, Jingfan Xiao, Qin Liu, Haizhen Wu, Lili Xu,Yuanxing Zhang* (2009) Edwardsiella tarda T6SS componentevpP is regulated byesrB and iron, and plays essential roles in the invasion of fish. Fish and Shellfish Immunology27(3): 469-477.
49.Yuanxing Zhang (2009) Approaches to optimizing animal cell culture process: substrate metabolism regulation and protein expression improvement. Advances in Biochemical Engineering/Biotechnology113: 177-215.
50.Qiyao Wang, Zhao Yang, Qin Liu*,Yuanxing Zhang* (2009) Surface-exposed expression ofEdwardsiella tarda EseB in live attenuatedVibrio anguillarum based on novel surface display systems. Aquaculture Research40(13):1459-1467.
51.JingfanXiao, Qiyao Wang, Qin Liu, Xin Wang, Huan Liu,Yuanxing Zhang* (2009) Isolation and identification of fish pathogenEdwardsiella tarda from mariculture in China. Aquaculture Research40(1):13-17.
52.Menghao Cai, Xiangshan Zhou*,Xueqian Sun, Kejing Tao,Yuanxing Zhang* (2009) Statistical optimization of medium composition for aspergiolide A production by marine-derived fungusAspergillus glaucus. Journal of Industrial Microbiology & Biotechnology36(3):381-389.
53.JingfanXiao, Qiyao Wang*, Qin Liu, Lili Xu, Xin Wang, Haizhen Wu,Yuanxing Zhang* (2009) Characterization ofEdwardsiella tardarpoS: its roles in controlling serum resistance, chondroitinase activity, biofilm formation, and autoinducer synthetases expression. Applied Microbiology and Biotechnology83(1):151-160.
54.WeiWei, Hongyan Xu*,Qiyao Wang, Xiao Zhang, Kangmei Chang, Changwen Wu,Yuanxing Zhang* (2009) Identification of differentially expressed genes in large yellow croaker (Pseudosciaena crocea) induced by attenuated liveVibrio anguillarum. Aquaculture291(1-2):124-129.
55.Xueqian Sun, Xiangshan Zhou*, Menghao Cai, Kejing Tao,Yuanxing Zhang* (2009) Identified biosynthetic pathway of aspergiolide A and a novel strategy to increase its production in a marine-derived fungusAspergillus glaucus by feeding of biosynthetic precursors and inhibitors simultaneously. Bioresource Technology100(18):4244-4251.
56.Jing Ye, Yue Ma, Qin Liu, Dongling Zhao,Qiyao Wang,Yuanxing Zhang* (2008) Regulation ofVibrio alginolyticus virulence by the LuxS quorum-sensing system. Journal of Fish Diseases31(3):161-169.
57.Chun Wei, Xiangshan Zhou,Yuanxing Zhang* (2008) Improving intracellular production of recombinant protein inPichia pastoris using an optimized preinduction glycerol-feeding scheme. Applied Microbiology and Biotechnology78(2):257-264.
58.Zheyong Ma, Xiaoping Yi,Yuanxing Zhang* (2008) Enhanced intracellular accumulation of recombinant HBsAg in CHO cells by dimethyl sulfoxide. Process Biochemistry43(6):690-695.
59.Weiyuan Zhang, Xiaoping Yi, Xiangming Sun,Yuanxing Zhang* (2008) Surface modification of non-woven poly (ethylene terephthalate) fabric scaffold for improving cell attachment in animal cell culture. Journal of Chemical Technology and Biotechnology83(6):904-911.
60.Yang Tian, Qiyao Wang, Qin Liu, Yue Ma, Xiaodan Cao, Lingyu Guan,Yuanxing Zhang* (2008) Involvement of LuxS in the regulation of motility and flagellar biogenesis inVibrio alginolyticus. Bioscience, Biotechnology, and Biochemistry72(4): 1063-1071.
61.Lingyun Zhou, Qin Liu*, Qiyao Wang, Yue Ma, Yuzhou Xu, Zhao Yang, Yan Zhao,Yuanxing Zhang* (2008) Secretary delivery of heterologous proteins in attenuatedVibrio anguillarum for potential use in vaccine design. Applied Microbiology and Biotechnology79(6):1027-1034.
62.Zhao Yang, Qin Liu,Qiyao Wang,Yuanxing Zhang* (2008) Gene cloning and expression of major capsid protein of large yellow croaker iridovirus and its secretion based ona-haemolysin transport system. Journal of the World Aquaculture Society39(6):774-780.
63.Zhao Yang, Qin Liu*,Qiyao Wang,Yuanxing Zhang* (2008) Novel bacterial surface display systems based on outer membrane anchoring elements from the marine bacteriumVibrio anguillarum. Applied and Environmental Microbiology74(14):4359-4365.
64.Ting Xie, Qin Liu*, Fusheng Xie, Haifeng Liu,Yuanxing Zhang* (2008) Secretory expression of insulin precursor inPichia pastoris and simple procedure for producing recombinant human insulin. Preparative Biochemistry and Biotechnology38(3):308-317.
65.Haopeng Rui, Qin Liu, Yue Ma,Qiyao Wang,Yuanxing Zhang* (2008) Roles of LuxR in extracellular alkaline serine protease A expression, extracellular polysaccharide production and mobility ofVibrio alginolyticus. FEMS Microbiology Letters285(2):155-162.
66.Yuzhou Xu, Qin Liu*, Lingyun Zhou, Zhao Yang,Yuanxing Zhang* (2008) Surface display of GFP byPseudomonas syringae truncated ice nucleation protein in attenuatedVibrio anguillarum strain. Marine Biotechnology10(6):701-708.
67.Yang Tian,Qiyao Wang, Qin Liu, Yue Ma, Xiaodan Cao,Yuanxing Zhang* (2008) Role of RpoS in stress survival, synthesis of extracellular autoinducer 2, and virulence inVibrio alginolyticus. Archives of Microbiology190(5): 585-594.
68.Qiyao Wang, Qin Liu, Xiaodan Cao, Minjun Yang,Yuanxing Zhang* (2008) Characterization of two TonB systems in marine fish pathogen Vibrio alginolyticus: their roles in iron utilization and virulence. Archives of Microbiology190(5): 595-603.
69.Wenying Wang, Xiaoping Yi,Yuanxing Zhang* (2007) Gene transcription acceleration: main cause of hepatitis B surface antigen production improvement by dimethyl sulfoxide in the culture of Chinese hamster ovary cells. Biotechnology and Bioengineering97(3):526-535.
70.Hongzhong Wu, Fan Yang, Shuya Cui, Yufeng Qin, Jianwen Liu*,Yuanxing Zhang* (2007) Hematopoietic effect of fractions from the enzyme-digestedcolla corii asini on mice with 5-fluorouracil induced anemia. The American Journal of Chinese Medicine35(5): 853-866.
71.Lu Yang, Yue Ma,Yuanxing Zhang* (2007) Freeze-drying of live attenuatedVibrio anguillarum mutant for vaccine preparation. Biologicals35(4):265-269.
72.Xiangming Sun,Yuanxing Zhang* (2007) A cell-detaching reactor for inoculation of anchorage-dependent CHO and Vero cells between stepwise-expanded bioreactors. Biotechnology Letters29(5): 697-701.
73.Qin Liu, Pengbo Wang, Yue Ma,Yuanxing Zhang* (2007) Characterization ofVibrio alginolyticusfur gene and localization of essential amino acid sites in Fur by in vitro site-directed mutagenesis. Journal of Molecular Microbiology and Technology13(1-3):15-21.
74.Liming Ouyang, Ling Ou,Yuanxing Zhang* (2007) An integrated teaching strategy for teaching biochemistry to biotechnology specialty students. Biochemistry and Molecular Biology Education35 (4): 267-271.
75.Qiyao Wang, Qin Liu, Yue Ma, Haopeng Rui,Yuanxing Zhang* (2007) LuxO controls extracellular protease, hemolytic activities and siderophore production in fish pathogenVibrio alginolyticus. Journal of Applied Microbiology103(5):1525-1534.
76.Qiyao Wang, Qin Liu, Yue Ma,Yuanxing Zhang* (2007) Isolation, sequencing and characterization of cluster genes involved in the biosynthesis and transportation of the siderophore of marine fish pathogenVibrio alginolyticus. Archives of Microbiology188(4):433-439.
77.Haiyan Huang, Yihua Yu, Xiaoping Yi,Yuanxing Zhang* (2007) Nitrogen metabolism of asparagine and glutamate in Vero cells studied by1H/15N NMR spectroscopy. Applied Microbiology and Biotechnology77(2):427-436.
78.Fang Zhang, Xiangming Sun, Xiaoping Yi,Yuanxing Zhang* (2006) Metabolic characteristics of recombinant Chinese hamster ovary cells expressing glutamine synthetase in presence and absence of glutamine. Cytotechnology51(1):21-28.
79.Anfeng Xiao, Xiangshan Zhou, Li Zhou,Yuanxing Zhang* (2006) Improvement of cell viability and hirudin production by ascorbic acid inPichia pastoris fermentation. Applied Microbiology and Biotechnology72(4): 837-844.
80.Haiyan Huang, Xiaoping Yi,Yuanxing Zhang* (2006) Improvement of Vero cell growth in glutamate-based culture by supplementing ammoniagenic compounds. Process Biochemistry41(12):2386-2392.
81.Hechun Zhang, Jixun Zhan, Keman Su,Yuanxing Zhang* (2006)A kind of potential food additive produced byStreptomyces coelicolor: characteristics of blue pigment and identification of a novel compound,l-actinorhodin.Food Chemistry95(2):186-192.
82.Junhui Li, Zhengyu Huang, Xiangming Sun, Pengyuan Yang,Yuanxing Zhang* (2006)Understanding the enhanced effect of dimethyl sulfoxideon hepatitis B surface antigenexpression in the culture ofChinese hamster ovary cells on the basis ofproteome analysis. Enzyme and Microbiol Technology38(3-4):372-380.
83.Junhui Li, Xiangming Sun,Yuanxing Zhang* (2006) Improvement of hepatitis B surface antigen expression by dimethyl sulfoxide in the culture of recombinant Chinese hamster ovary cells. Process Biochemistry41(2):317-322.
84.Qin Liu, Yue Ma, Lingyun Zhou,Yuanxing Zhang* (2006)Gene cloning, expression and functional characterization of an acyl carrier protein AcpV fromVibrio anguillarum.Archives of Microbiology185(2):159-163.
85.Haifeng Liu, Xiangshan Zhou,Yuanxing Zhang* (2006) A comparative investigation on different refolding strategies of recombinant human tissue-type plasminogen activator derivative. Biotechnology Letters28(7):457-463.
86.Qin Liu, Yue Ma, Lingyun Zhou,Yuanxing Zhang* (2005) Gene cloning and functional characterization of a phosphopantetheinyl transferase fromVibrio anguillarum serotype O1. Achieves of Microbiology183(1):37-44.
87.Subiao Lu, Xiangming Sun,Yuanxing Zhang* (2005) Insight into metabolism of CHO cells at low glucose concentration on the basis of the determination of intracellular metabolites. Process Biochemistry40(5):1917-1921.
88.Juxing Chen, Xiangming Sun,Yuanxing Zhang* (2005) Growth and metabolism of marine fish Chinook salmon embryo cells: Response to lack of glucose and glutamine. Biotechnology Letters27(6): 395-401.
89.Juxing Chen, Xiangming Sun, Li Zhang, Xiaoping Yi,Yuanxing Zhang* (2005) Mass cultivation of marine fish Chinook salmon embryo cells in bioreactor with low-serum medium. Aquaculture249(1-4):35-45.
90.Mingfei Shao, Yue Ma, Qin Liu,Yuanxing Zhang* (2005) Secretory expression of recombinant proteins in an attenuatedVibrio anguillarum strain for potential use in vaccines. Journal of Fish Diseases28(6):723-
91.Xiangming Sun,Yuanxing Zhang* (2004) Glutamine cannot support recombinant CHO cell growth and maintenance in the absence of glucose. Process Biochemistry39(6):719-722.
92.Li Zhang, Hong Shen,Yuanxing Zhang* (2004) Fed-batch culture of hybridoma cells in serum-free medium using an optimized feeding strategy. Journal of Chemical Technology and Biotechnology79(2):171-181.
93.Xiaoping Yi, Xiangming Sun,Yuanxing Zhang* (2004) Effects of osmotic pressure on recombinant BHK cell growth and von willebrand factor (vWF) expression. Process Biochemistry39(11):1817-1823.
94.Qin Liu, Yue Ma, Haizhen Wu, Mingfei Shao, Haifeng Liu,Yuanxing Zhang* (2004) Cloning, identification and expression of anentE homologue angE fromVibrio anguillarum serotype O1. Archives of Microbiology181(4):287-293.
95.Weibin Zhou,Yuanxing Zhang* (2004) Purification and identification of recombinant hirudin and its degradation derivatives expressed inPichia pastoris. Preparative Biochemistry & Biotechnology34(3):239-252.
96.Jizhong Yang, Xiangshan Zhou,Yuanxing Zhang* (2004) Improvement of recombinant hirudin production by controlling NH4+ concentration inPichia pastoris fermentation. Biotechnology Letter26(12):1013-1017.
97.Yuanxing Zhang*, Lin Dai, Xiangming Sun (2003) Kinetic aspect of hepatitis B surface antigen production in recombinantSaccharomyces cerevisiae fermentation. Process Biochemistry38(11):1593-1598.
98.Xiangming Sun,Yuanxing Zhang* (2003) Estimation of cell density in packed-bed bioreactor by lactate production rate. Biotechnology Letters25(11):853-857.
99.Subiao Lu, Xiangming Sun, Chaoou Shi,Yuanxing Zhang* (2003) Determination of tricarboxylic acid cycle acids and other relatedsubstances in cultured mammalian cells by gradient ion-exchange chromatography with suppressed conductivity detection. Journal of Chromatography A1012(2):161-168.
100.Xiangshan Zhou, Jian Lu, Weimin Fan,Yuanxing Zhang* (2002) Development of a responsive methanol sensor and its application in Pichia pastoris fermentation. Biotechnology Letters24(8): 643-646.
101.Xiangshan Zhou,Yuanxing Zhang* (2002) Decrease of proteolytic degradation of recombinant hirudin produced byPichia pastoris by controlling the specific growth rate. Biotechnology Letters24(17):1449-1453.
102.Jingli Xie,Yuanxing Zhang*, Yonghong Wang, Yuanguang Li (2001) Mixotrophic cultivation ofPlatymonas subcordiformis. Journal of Applied Phycology13(4):343-347.
103.Xiangming Sun,Yuanxing Zhang*, Wensong Tan, Yajing Zhou, Ping Hua (2000) Attachment kinetics of Vero cells onto CT3 microcarriers. Journal of Bioscience and Bioengineering90(1):32-36.
联系方法：上海市梅陇路130号4688美高梅集团唯一，200237
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